Composite

Part:BBa_M36475:Design

Designed by: Tamu Adumer and Yaya Odei   Group: Stanford BIOE44 - S11   (2014-10-23)


Codes for AraC, a transcription factor which represses operon. Arabinose dislodges TF protein.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1224
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1059
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1041


Design Notes

The terminator and AraC gene are coded for on the anti sense strand while the rest of the gene is coded for on the sense strand.


Source

It is from a genomic sequence

References